Erg mediates downregulation of claudin‐5 in the brain endothelium of a murine experimental model of cerebral malaria. Issue 18 (17th July 2019)

• Record Type: Journal Article
• Authors: Liu, Fuhong; Liu, Qiang; Yuan, Fangshu; Guo, Shuling; Liu, Jinzhi; Sun, Zongguo; Gao, Peng; Wang, Yu; Yan, Suhua; Liu, Ju
• Is Part Of: FEBS letters. Volume 593:Issue 18(2019)
• Page Start: 2585
• Page End: 2595
• Publication Date: 2019-07-17
• Subjects: cerebral malaria -- claudin‐5 -- Erg -- permeability -- transcriptional regulation; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13526
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Cerebral malaria (CM) is a severe complication with brain vascular hyperpermeability. Claudin‐5 is the major component of tight junctions. To investigate the expression of claudin‐5 in CM, we established a murine experimental cerebral malaria (ECM) model and an in vitro model by treating murine brain endothelial cells (bEnd3) with plasma from ECM mice. Expression of claudin‐5 and the ETS transcription factor Erg was reduced in the brain endothelium of ECM mice. In bEnd3 cells exposed to ECM plasma, decreased expression of claudin‐5 and Erg, and increased permeability were observed. Silencing of Erg significantly reduced Cldn5 expression. ChIP assays indicated that Erg binds to the −813 ETS motif of the murine Cldn5 gene promoter, and the binding is decreased by treatment with ECM plasma. Abstract :