In vitro & in silico study of hypoglycemic potential of Pterocarpus marsupium heartwood extract. Issue 22 (17th November 2019)

• Record Type: Journal Article
• Authors: Yadav, Vivek Kumar; Mishra, Abha
• Is Part Of: Natural product research. Volume 33:Issue 22(2019)
• Page Start: 3298
• Page End: 3302
• Publication Date: 2019-11-17
• Subjects: Pterocarpus marsupium -- α-amylase -- α-glucosidase -- antioxidants -- In vitro -- In-silico; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1471078
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Total amount of phenols, flavonoids and tannins in methanolic extract of heartwood Pterocarpus marsupium were found to be 124.4 ± 4.6, 485.5 ± 2.7, and 71.6 ± 4.2 mg/gm of plant extract respectively. HPLC analyses of extract showed retention time at 6.2 min and FTIR analysis confirmed presence of Liquiritigenin in methanolic extract. In DPPH scavenging activity and ABTS∙+ scavenging activity, IC50 values were found to be 138.3, 12.4, 13.5 and 47.8, 3.9, 4.2 μg/mL for aqueous, methanolic extract and standard ascorbic acid respectively. In α-amylase inhibition assay and α-glucosidase inhibition assay, IC50 values for standard Acarbose, aqueous and methanolic extract of heartwood were 44.09, 166.72, 48.20 and 45.17, 172.32, 48.12 respectively. Molecular docking study showed hydrogen bonding between Liquiritigenin and catalytic triad (Asp197, Glu233, and Asp300) of α-amylase and His407 of α –Glucosidase with −5.60 and −7.10 binding energies respectively. Abstract :