Involvement of SAPK/JNK Signaling Pathway in Copper Enhanced Zinc-Induced Neuronal Cell Death. (15th February 2019)

• Record Type: Journal Article
• Authors: Tanaka, Ken-Ichiro; Shimoda, Mikako; Kasai, Misato; Ikeda, Mayumi; Ishima, Yu; Kawahara, Masahiro
• Is Part Of: Toxicological sciences. Volume 169:Number 1(2019)
• Page Start: 293
• Page End: 302
• Publication Date: 2019-02-15
• Subjects: zinc -- copper -- neurotoxicity -- SAPK/JNK -- ER stress -- oxidative stress; Toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology; Periodicals; 615.9
• DOI: 10.1093/toxsci/kfz043
• Languages: English
• ISSNs: 1096-6080

Abstract

Abstract: Zinc (Zn) plays an important role in many organisms in various physiological functions such as cell division, immune mechanisms and protein synthesis. However, excessive Zn release is induced in pathological situations and causes neuronal cell death. Previously, we reported that Cu ions (Cu 2+ ) markedly exacerbates Zn 2+ -induced neuronal cell death by potentiating oxidative stress and the endoplasmic reticulum stress response. In contrast, the stress-activated protein kinase/c-Jun amino-terminal kinase (SAPK/JNK) signaling pathway is important in neuronal cell death. Thus, in this study, we focused on the SAPK/JNK signaling pathway and examined its involvement in Cu 2+ /Zn 2+ -induced neurotoxicity. Initially, we examined expression of factors involved in the SAPK/JNK signaling pathway. Accordingly, we found that phosphorylated (ie, active) forms of SAPK/JNK (p46 and p54) are increased by CuCl2 and ZnCl2 co-treatment in hypothalamic neuronal mouse cells (GT1-7 cells). Downstream factors of SAPK/JNK, phospho-c-Jun, and phospho-activating transcription factor 2 are also induced by CuCl2 and ZnCl2 co-treatment. Moreover, an inhibitor of the SAPK/JNK signaling pathway, SP600125, significantly suppressed neuronal cell death and activation of the SAPK/JNK signaling pathway induced by CuCl2 and ZnCl2 cotreatment. Finally, we examined involvement of oxidative stress in activation of the SAPK/JNK signaling pathway, and found that human serum albumin-thioredoxin fusion protein, an antioxidative protein, suppresses activation of the SAPK/JNK signaling pathway. On the basis of these results, our findings suggest that activation of ZnCl2 -dependent SAPK/JNK signaling pathway is important in neuronal cell death, and CuCl2 -induced oxidative stress triggers the activation of this pathway.