An MLST approach to support tracking of plasmids carrying OXA-48-like carbapenemase. (24th April 2019)

• Record Type: Journal Article
• Authors: Brehony, Carina; McGrath, Elaine; Brennan, Wendy; Tuohy, Alma; Whyte, Thomas; Brisse, Sylvain; Maiden, Martin; Jolley, Keith; Morris, Dearbháile; Cormican, Martin
• Is Part Of: Journal of antimicrobial chemotherapy. Volume 74:Number 7(2019)
• Page Start: 1856
• Page End: 1862
• Publication Date: 2019-04-24
• Subjects: Anti-infective agents -- Periodicals; Chemotherapy -- Periodicals; 615.58
• DOI: 10.1093/jac/dkz136
• Languages: English
• ISSNs: 0305-7453

Abstract

Abstract: Objectives: The prevalence of infections caused by OXA-48-like carbapenemase-producing organisms in Ireland has increased dramatically since 2011 and is an urgent public health issue. Genome-based high-resolution genotyping was used to analyse clinical isolates submitted to the Irish Carbapenemase-Producing Enterobacteriaceae Reference Laboratory Service for a 13 month period (2016–17). Methods: A total of 109 OXA-48-producing non-duplicate clinical isolates from 16 submitting centres were sequenced. Using a gene-by-gene approach, isolate genomes were characterized by MLST and core genome MLST, and the presence of antimicrobial resistance determinants was determined. Reference mapping and a novel plasmid MLST-type approach was applied to determine plasmid background. Results: The OXA-48-like-producing isolates were Escherichia coli ( n = 56), Klebsiella spp. ( n = 46) and Enterobacter cloacae ( n = 7). Amongst the E. coli isolates there were 37 different STs and amongst the Klebsiella spp. isolates there were 27 different STs. bla OXA-48 was present in 105/109 (96.3%) of isolates. Based on mapping analysis and detection of the pOXA-48 IncL-type plasmid replicon and backbone genes, a pOXA-48-like plasmid was identified in 93/109 isolates (85.3%). The remaining isolates ( n = 16; 14.7%) harboured bla OXA-48 -like genes in unknown environments. Using a gene-by-gene approach two pOXA-48-like plasmid groups with 2/71 pOXA-48-like locus differences between them were identified. Conclusions: In Ireland we found a diversity of genotypes associated with OXA-48-like-producing clinical isolates with the IncL pOXA-48 plasmid type predominating as the bla OXA-48 genetic environment. A plasmid MLST approach can rapidly identify plasmids associated with outbreaks and monitor spread of types temporally and geographically.