Alkaloids of NIRAM, natural dye from Polygonum tinctorium, and their anti-inflammatory activities. Issue 41 (10th October 2019)

• Record Type: Journal Article
• Authors: Kim, Dong Hyun; Kim, Chung Sub; Subedi, Lalita; Kim, Sun Yeou; Lee, Kang Ro
• Is Part Of: Tetrahedron letters. Volume 60:Issue 41(2019)
• Publication Date: 2019-10-10
• Subjects: NIRAM -- Polygonum tinctorium -- Indole alkaloids -- Anti-Inflammatory activities; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tetlet.2019.151130
• Languages: English
• ISSNs: 0040-4039

Abstract

Graphical abstract: Highlights: Four new indole alkaloids and five known alkaloids were isolated. The isolated compounds were elucidated by spectroscopic and computational methods. Compounds1, 2, 3, 5 and7 showed potent NO production inhibitory activities. Abstract: Phytochemical investigation of EtOH extract of NIRAM, natural dye from Polygonum tinctorium, resulted in the purification of nine alkaloid compounds (1 –9 ) including four new compounds (1 –4 ). Structures of these new compounds were elucidated by 1D and 2D NMR ( 1 H and 13 C NMR, 1 H– 1 H COSY, 1 H– 13 C HSQC, 1 H– 13 C HMBC), IR, UV, HR-ESI-MS, and ECD spectra. Isolated compounds (1 –9 ) were tested for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 cells. Compounds1 –3, 5, and7 showed potent NO production inhibitory activities, with IC50 values of 3.88–22.87 μ M.