Regulatory T cells and CD20+ B cells in pediatric very severe aplastic anemia: possible clinical markers for evaluating the therapeutic efficacy and prognosis. Issue 10 (26th November 2018)

• Record Type: Journal Article
• Authors: Fang, Junyue; Lin, Li; Wang, Ying; Lin, Dijin; Liu, Chunyue; Sunlong, Qiaozi; Lin, Xianghua
• Is Part Of: Hematology. Volume 23:Issue 10(2018)
• Page Start: 823
• Page End: 827
• Publication Date: 2018-11-26
• Subjects: Very severe aplastic anemia -- pediatrics -- CD20+ B cells -- Tregs; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; 616.15005
• DOI: 10.1080/10245332.2018.1498566
• Languages: English
• ISSNs: 1024-5332

Abstract

ABSTRACT: Objectives: To investigate the immune status of children with very severe aplastic anemia (VSAA), and evaluate the frequencies of CD20 + B cells and Regulatory T cells (Tregs) as potential markers for evaluating the therapeutic efficacy and prognosis. Methods: We systematically analyzed CD20 + B cells and Tregs using Flow Cytometry in 36 children with VSAA (14 newly diagnosed cases and 22 cases in remission after therapy with HDIVIG + r-ATG + CSA). Results: In newly diagnosed VSAA patients, the percentage of CD20 + B cells was higher than that in healthy children ( P < .01), whereas the percentage of Tregs was lower than that in healthy children ( P < .001). After treatment with HDIVIG + r-ATG + CSA, the percentage of CD20 + B cells in peripheral blood was decreased obviously, and the percentage of Tregs was significantly increased. Conclusion: There is a moderate negative correlation between the percentage of Tregs and CD20 + B cells in our study. Our results shed light on the roles of Tregs and CD20 + B cells as therapeutic efficacy and prognostic markers of pediatric VSAA. Moreover, the mechanism underlying the decrease of blood Tregs and increase of CD20 + B cells in pediatric VSAA patients have been discussed, indicating that Tregs may suppress B cell responses.