A simple and sensitive two-tube multiplex PCR assay for simultaneous detection of ten meat species. (March 2018) - Prusakova, Olga V.
Abstract: A sensitive and reliable technique for meat species identification is required to prevent food adulteration, particularly in meat production. This work developed an optimized multiplex PCR assay for simultaneous identification of five commonly consumed and five commonly banned meat species in meat products. We designed primers that specifically amplified mitochondrial ATPase subunit 8 gene regions of different lengths of bovine, ovine, swine, chicken, turkey, cat, dog, mouse and human DNAs. The developed multiplex PCR assay proved to be specific, sensitive down to 30 pg DNA per reaction, reproducible and economical. It could be used with a variety of raw meats and processed food samples and is easily applicable in a routine laboratory analysis without specific sophisticated equipment. Highlights: A multiplex PCR assay for simultaneous detection of ten meat species was developed. The assay was shown to be applicable for raw, boiled and microwave-cooked meats. The assay can be used in routine laboratory analyses without sophisticated equipment. A limited analysis of meat products on the Russian market was made. Over 90% of the tested products were found to be adulterated.