Anti-biofilm activity of A22 ((S-3, 4-dichlorobenzyl) isothiourea hydrochloride) against Pseudomonas aeruginosa: Influence on biofilm formation, motility and bioadhesion. (October 2017)

• Record Type: Journal Article
• Authors: Bonez, Pauline Cordenonsi; Rossi, Grazielle Guidolin; Bandeira, Jardel Rodrigo; Ramos, Andiara Prates; Mizdal, Caren Rigon; Agertt, Vanessa Albertina; Dalla Nora, Eloísa Salete Segatto; de Souza, Márcia Ebling; dos Santos Alves, Camilla Fillippi; dos Santos, Fallon Siqueira; Gündel, André; de Almeida Vaucher, Rodrigo; Santos, Roberto Christ Vianna; de Campos, Marli Matiko Anraku
• Is Part Of: Microbial pathogenesis. Volume 111(2017)
• Page Start: 6
• Page End: 13
• Publication Date: 2017-10
• Subjects: A22 -- Adhesion -- Bacterial motility -- Biofilms -- Pseudomonas aeruginosa -- Surfaces; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.08.008
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Bacterial biofilms are involved in various medical infections and for this reason it is of great importance understanding adhesion mechanisms of involved microorganisms is essential to develop new strategies of prevention and control. Different approaches have been used for preventing biofilm related infections in health care settings, such as use of surface coatings agents in medical implants. In this context, is necessary to explore new compounds with anti-biofilm activity. Thus, this study evaluated for the first time the action of A22 against biofilms of Pseudomonas aeruginosa PAO1 strain and multi-resistant clinical isolates on biotic and abiotic surfaces. A22 acts as inhibitor of the MreB protein of the bacterial cell wall, causing the rods to change shape to the coccoid form. In this work, A22 at subinhibitory concentrations was able to prevent biofilm formation, and atomic force microscopy images showed that A22 was highly effective in inhibiting adhesion on polyethylene surfaces. Pseudomonas aeruginosa PAO1 exhibited a strong ability to adhere to HeLa cells, and A22 inhibited the aggregation after 4 h of exposure. Swarming and twitching motilities were significantly altered by A22 at subinhibitory concentrations. Thus, by changing the shape of the bacterial cell, many properties can be affected, such as motility, surface adhesion and biofilm formation. This work presents A22 as a promising novel antibacterial or surface coating agent of medical materials. Highlights: Swarming and twitching motilities were impaired by A22. A22 was able to prevent biofilm formation of MDR microorganisms. A22 inhibited the aggregation of P. aeruginosa PAO1 bacilli to HeLa cells. The MreB appears as a new therapeutic target to combat the biofilm formation. A22 was presented as a promising surface coating agent of medical materials.