Thiamine accumulation and thiamine triphosphate decline occur in parallel with ATP exhaustion during postmortem aging of pork muscles. (March 2018)

• Record Type: Journal Article
• Authors: Muroya, S.; Oe, M.; Ojima, K.
• Is Part Of: Meat science. Volume 137(2018)
• Page Start: 228
• Page End: 234
• Publication Date: 2018-03
• Subjects: Loin -- Muscle type -- Pork -- Postmortem aging -- Thiamine triphosphate -- Vitamin B1; AK1 adenylate kinase isoform 1 -- CE-TOFMS capillary electrophoresis-time of flight mass spectrometry -- LL longissimus lumborum -- MyHC myosin heavy chain -- qPCR quantitative PCR -- RPL7 Ribosomal protein L7 (official gene symbol) -- ThDP thiamine diphosphate -- ThDPase thiamine diphosphatase (enzyme) -- ThMP thiamine monophosphate -- THTPA thiamine triphosphatase (official gene symbol) -- ThTPase thiamine triphosphatase (enzyme) -- THTR1 thiamine transporter 1 (official gene symbol) -- THTR2 thiamine transporter 2 (official gene symbol) -- TPK1 thiamine pyrophosphokinase 1 (official gene symbol) -- ThTP thiamine triphosphate -- VI vastus intermedius; Meat -- Periodicals; Meat industry and trade -- Periodicals; Viande -- Périodiques; Viande -- Industrie -- Périodiques; Meat; Meat industry and trade; Periodicals; 641.36
• DOI: 10.1016/j.meatsci.2017.11.035
• Languages: English
• ISSNs: 0309-1740

Abstract

Abstract: We aimed to clarify the mechanisms affecting postmortem thiamine and its phosphoester contents in major edible pork muscles, namely the longissimus lumborum (LL) in addition to vastus intermedius (VI). Metabolomic analysis by capillary electrophoresis-time of flight mass spectrometry revealed that the level of thiamine triphosphate (ThTP), approximately 1.8-fold higher in LL than in VI muscle at 0 h postmortem, declined in the first 24 hrs, resulting in an undetectable level at 168 h postmortem in both muscles. In contrast, the thiamine content in both muscles increased after 24 h postmortem during the aging process. The thiamine accumulation and ThTP decline progressed in parallel with a drastic reduction of the ATP level. The intermuscular differences in pH at 24 h and in expression of thiamine transporter and thiamine pyrophosphokinase might result in delayed thiamine generation in LL. These results suggest that postmortem ATP exhaustion forced ThTP hydrolysis and further depyrophosphorylation of thiamine diphosphate in the porcine muscles, which resulted in thiamine accumulation. Highlights: Levels of ThTP and ATP decline in parallel during postmortem pig longissimus muscle. Thiamine level in pig longissimus and vastus intermedius increases in the duration. Changes in thiamine and its phosphate levels were different between the muscles. Expressions of genes related to thiamine metabolism differ between the two muscles.