Phosvitin and its hydrolysate promote differentiation and inhibit TNF-ɑ induced inflammation in MC3T3-E1 cells via ERK and AKT pathways. (February 2019)

• Record Type: Journal Article
• Authors: Ren, Jiandong; Chakrabarti, Subhadeep; Wu, Jianping
• Is Part Of: Journal of functional foods. Volume 53(2019)
• Page Start: 259
• Page End: 265
• Publication Date: 2019-02
• Subjects: Phosvitin -- MC3T3-E1 -- Differentiation -- RUNX2 -- Inflammation -- Signaling pathway; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.12.025
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Phosvitin and phosvitin hydrolysate promote MC3T3-E1 cells differentiation. Expression of RUNX2, alkaline phosphatase, osteocalcin and collagen were upregulated. Phosvitin and phosvitin hydrolysate inhibit TNF-α induced inflammation in MC3T3-E1. Phosvitin and phosvitin hydrolysate activate ERK and AKT signaling pathways. Abstract: Phosvitin and its hydrolysate were reported to show antioxidant, anti-inflammation and antimicrobial activities. Given the critical role of inflammation on bone health, the work was designed to study the effects of phosvitin and its hydrolysate on osteoblast differentiation and inflammation in osteoblastic (MC3T3-E1) cells. Both phosvitin and its hydrolysate promoted osteoblast differentiation by upregulating expression of runt-related transcription factor 2, alkaline phosphatase (by phosvitin hydrolysate), osteocalcin production (by phosvitin) and collagen synthesis. They also inhibited TNF-ɑ induced inflammation by reducing the production of regulated on activation, normal T cell expressed and secreted and monocyte chemoattractant protein-1, which are crucial for recruiting osteoclast progenitor and maintaining osteoclast functions, and cyclooxygenase-2, a proinflammatory protein. Adding phosvitin and its hydrolysate activated both extracellular signal–regulated kinases (ERK) and protein kinase B (AKT) signaling pathways, indicating that both phosvitin and its hydrolysate promote osteoblast differentiation and exert antiinflammation effects via ERK and AKT pathways.