Polyphenol-rich ethyl acetate fraction of Molineria latifolia rhizome restores oxidant-antioxidant balance by possible engagement of KEAP1-NRF2 and PKC/NF-κB signalling pathways. (March 2018)

• Record Type: Journal Article
• Authors: Ooi, Der Jiun; Chan, Kim Wei; Ismail, Norsharina; Imam, Mustapha Umar; Ismail, Maznah
• Is Part Of: Journal of functional foods. Volume 42(2018)
• Page Start: 111
• Page End: 121
• Publication Date: 2018-03
• Subjects: EAF ethyl acetate fraction -- NC normal control -- DC diabetic control -- GC glibenclamide-treated diabetic control -- T2DM type 2 diabetes mellitus; Ethyl acetate fraction -- Oxidant-antioxidant balance -- Diabetes mellitus -- KEAP1-NRF2 signalling -- PKC/NF-κB signalling; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.11.044
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: The chemical compositions of EAF was analysed by non-targeted HPLC-QTOF mass spectrometry. Polyphenol-rich EAF restored diabetes-induced systemic oxidant-antioxidant imbalance. Polyphenol-rich EAF modulated tissue-specific antioxidant responses. Transcriptional analysis revealed concerted modulation at the NRF2–NF-κB axis. Polyphenol-rich EAF warrants potential pharmaceutical/nutraceutical applications. Abstract: The present study examined the potential of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome to modulate the oxidant-antioxidant balance in high fat diet and nicotinamide/streptozotocin-induced diabetic rats. Administration with EAF ameliorated systemic antioxidant status- and oxidative stress-related parameters without affecting the renal and liver functions in the diabetic rats. Further analyses on adipose, muscle and liver tissues demonstrated differing ability to scavenge free radicals and protection against lipid peroxidation. Transcriptional changes proposed concerted modulation of both KEAP1-NRF2 and PKC/NF-κB signallings in tissue-specific manner. Qualitative profiling of compounds present in EAF was analysed by non-targeted HPLC-QTOF mass spectrometry. A total of 23 unique mass signals were detected in EAF. Putative identification revealed a mixture of naturally occurring polyphenols ranging from cinnamic acid-, benzoic acid- and flavonoid-derived groups. Overall, the study demonstrated potential application of EAF to reinstate diabetes-induced oxidant-antioxidant imbalance by potentially modulating the NRF2–NF-κB signalling axis.