ROS-modulating anticancer effects of gastrointestinally digested Ribes nigrum L. fruit extract in human colon cancer cells. (March 2018)

• Record Type: Journal Article
• Authors: Olejnik, Anna; Kaczmarek, Mariusz; Olkowicz, Mariola; Kowalska, Katarzyna; Juzwa, Wojciech; Dembczyński, Radosław
• Is Part Of: Journal of functional foods. Volume 42(2018)
• Page Start: 224
• Page End: 236
• Publication Date: 2018-03
• Subjects: Blackcurrant -- HT-29 cells -- Cell cycle -- Apoptosis -- Invasion -- Metalloproteinases; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.01.011
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Digested blackcurrant extract dose-dependently modulates ROS in colon cancer cells. Oxidant and antioxidant effects of the digested extract in colon cancer cells. The extract induces caspase 3-mediated apoptosis in colon cancer HT-29 cells. The extract evokes a G0 /G1 phase cell cycle arrest in colon cancer cells. The extract inhibits cell invasion by matrix metalloproteinase suppression. Abstract: Reactive oxygen species (ROS) and antioxidants play a crucial role in dose-dependent promotion and suppression of colorectal tumourigenesis. In this study, ROS-modulating anticancer effects were analysed in colon cancer HT-29 cells exposed to the blackcurrant (BC) extract digested in an artificial gastrointestinal tract. The obtained results indicate that the colon-available fraction of gastrointestinal-digested BC extract dose-dependently inhibited the growth of colon cancer cells by inducing a G0 /G1 phase cell cycle arrest and targeting cells to a caspase 3-mediated apoptosis pathway. The extract significantly suppressed cell invasion by down-regulation of matrix metalloproteinase MMP-2 and MMP-9 expression. The anticancer effect of the BC extract was related to the dose-dependent modulation of intracellular ROS accumulation. Given the bioavailability of BC polyphenols to the colon and their multi-directional anticancer activity, BC fruit may be proposed as a natural therapeutic agent and functional food ingredient to support prevention and treatment of colorectal cancer.