Berberine attenuates sodium palmitate-induced lipid accumulation, oxidative stress and apoptosis in grass carp(Ctenopharyngodon idella)hepatocyte in vitro. Issue 88 (May 2019)

• Record Type: Journal Article
• Authors: Yang, Shuo-Shuo; Yu, Cheng-Bing; Luo, Zhen; Luo, Wen-Li; Zhang, Jing; Xu, Jian-Xiong; Xu, Wei-Na
• Is Part Of: Fish & shellfish immunology. Issue 88(2019)
• Page Start: 518
• Page End: 527
• Publication Date: 2019-05
• Subjects: Berberine -- Sodium palmitate -- Hepatocyte -- Lipid accumulation -- Oxidative stress -- Apoptosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.02.055
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The objective of this work was to investigate the effect of berberine (BBR) on the Cell viability, lipid accumulation, apoptosis, cytochrome c, caspase-9 and caspase-3 in lipid accumulation-hepatocytes induced by sodium palmitate in vitro. The lipid accumulation-hepatocytes (induced by 0.5 mM sodium palmitate for 24 h) were treated with 5 μM berberine for 12 h. Then, the Cell viability, intracellular triglyceride (TG) content, lipid peroxide (LPO), malonaldehyde (MDA) content, cytochrome c, caspase-9, caspase-3 and apoptosis were detected. Sodium palmitate decreased Cell viability and increased intracellular TG content, lipid droplet accumulation, LPO and MDA concentrations, caused caspase-3 and caspase-9 activation, then led to apoptosis accompanied by cytochrome c release from mitochondria into the cytoplasm. Beberine could improve intracellular lipid droplet accumulation and oxidative stress, while reduce apoptosis induced by sodium palmitate. Highlights: Hepatocytes lipid accumulation. Berberine improves oxidative stress. Apoptosis.