Use of mucus as a non‐invasive sampling method for DNA barcoding of stingrays and skates (batoid elasmobranchs). Issue 3 (22nd February 2019)

• Record Type: Journal Article
• Authors: Domingues, Rodrigo R.; Garrone‐Neto, Domingos; Hilsdorf, Alexandre W. S.; Gadig, Otto B. F.
• Is Part Of: Journal of fish biology. Volume 94:Issue 3(2019)
• Page Start: 512
• Page End: 516
• Publication Date: 2019-02-22
• Subjects: cytochrome c oxidase subunit 1 -- endangered elasmobranchs -- molecular markers -- non‐lethal methods; Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.13919
• Languages: English
• ISSNs: 0022-1112

Abstract

Abstract : In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99–100% accuracy to the species level. This method proved to provide reliable and good‐quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.