A method to quantify genotoxicity of malathion in rainbow trout using the weighted averaging. (13th October 2018)

• Record Type: Journal Article
• Authors: Poorbagher, Hadi; Ghaffari Farsani, Hamed; Farahmand, Hamid
• Is Part Of: Toxicology mechanisms and methods. Volume 28:Number 8(2018)
• Page Start: 607
• Page End: 614
• Publication Date: 2018-10-13
• Subjects: Genetic toxicity -- pesticides -- biostatistics -- malathion -- weighted averaging; Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.1080/15376516.2018.1480079
• Languages: English
• ISSNs: 1537-6516

Abstract

Abstract: DNA breakage has been frequently used as a biomarker of the pesticide toxicity. The present study introduced a method to quantify the DNA breakage in Oncorhynchus mykiss exposed to the pesticide malathion. Specimens were exposed to different concentrations of malathion for 1–9 days and their gill and liver were sampled. DNA was extracted and electrophoresed using agarose gel. The pixel density curves were obtained from the gel smears. The area under the curves was arbitrarily divided from three up to seven segments using a Java macro in the software ImageJ. Some weighted averaging methods were used to calculate DNA breakage in each gel lane. Akaike information criterion (AIC) was used to find the best analysis of variance. The liver was more sensitive than the gill showing a larger number of significant differences among the specimens exposed to various concentrations of malathion. The geometric weighted averaging on the data extracted from the seven-segment pixel density curve resulted to the lowest AIC. The double-strand DNA breakage of O. mykiss was able to detect malathion in freshwater in concentrations over 0.05 mg L −1 .