Assessing the partners of the RBP9-mRNP complex in Trypanosoma cruzi using shotgun proteomics and RNA-seq. Issue 8 (3rd August 2018)

• Record Type: Journal Article
• Authors: Wippel, Helisa Helena; Inoue, Alexandre Haruo; Vidal, Newton Medeiros; Costa, Jimena Ferreira da; Marcon, Bruna Hilzendeger; Romagnoli, Bruno Accioly Alves; Santos, Marlon Dias Mariano; Carvalho, Paulo Costa; Goldenberg, Samuel; Alves, Lysangela Ronalte
• Is Part Of: RNA biology. Volume 15:Issue 8(2018)
• Page Start: 1106
• Page End: 1118
• Publication Date: 2018-08-03
• Subjects: RNA-binding protein -- gene expression regulation -- translation regulation -- Trypanosoma cruzi; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2018.1509660
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: Gene expression regulation in trypanosomes differs from other eukaryotes due to absence of transcriptional regulation for most of their genes. RNA-binding proteins (RBPs) associate with mRNAs and other regulatory proteins to form ribonucleoprotein complexes (mRNPs), which play a major role in post-transcriptional regulation. Here, we show that RBP9 is a cytoplasmic RBP in Trypanosoma cruzi with one RNA-recognition motif (RRM). The RBP9 sedimentation profile in a sucrose gradient indicated its presence in cytoplasmic translational complexes, suggesting its involvement in translation regulation. Taking this result as a motivation, we used shotgun proteomics and RNA-seq approaches to assess the core of the RBP9-mRNP complex. In epimastigotes in exponential growth, the complex was composed mostly by RBPs involved in RNA metabolism, such as ZC3H39, UBP1/2, NRBD1, and ALBA3/4. When parasites were subjected to nutritional stress, our analysis identified regulatory RBPs and the translation initiation factors eIF4E5, eIF4G5, eIF4G1, and eIF4G4. The RNA-seq results showed that RBP9-mRNP complex regulates transcripts encoding some RBPs – e.g. RBP5, RBP6, and RBP10 -, and proteins involved in metabolic processes. Therefore, we argue that RBP9 is part of cytoplasmic mRNPs complexes associated with mRNA metabolism and translation regulation in T. cruzi .