Exogenous expression of caveolin‐1 is sufficient for hepatic stellate cell activation. Issue 11 (30th June 2019)

• Record Type: Journal Article
• Authors: Ilha, Mariana; Moraes, Ketlen da Silveira; Rohden, Francieli; Martins, Leo Anderson Meira; Borojevic, Radovan; Lenz, Guido; Barbé‐Tuana, Florencia; Guma, Fátima Costa Rodrigues
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 11(2019)
• Page Start: 19031
• Page End: 19043
• Publication Date: 2019-06-30
• Subjects: caveolae -- caveolin‐1 -- GRX -- hepatic stellate cells activation -- liver fibrosis -- molecular marker; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.29226
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Caveolin‐1 (Cav‐1) expression is increased in hepatic stellate cells (HSC) upon liver cirrhosis and it functions as an integral membrane protein of lipid rafts and caveolae that regulates and integrates multiple signals as a platform. This study aimed to evaluate the role of Cav‐1 in HSC. Thus, the effects of exogenous expression of Cav‐1 in GRX cells, a model of activated HSC, were determined. Here, we demonstrated through evaluating well‐known HSC activation markers – such as α‐smooth muscle actin, collagen I, and glial fibrillary acidic protein – that up regulation of Cav‐1 induced GRX to a more activated phenotype. GRX EGFP‐Cav1 presented an increased migration, an altered adhesion pattern, a reorganization f‐actin cytoskeleton, an arrested cell cycle, a modified cellular ultrastructure, and a raised endocytic flux. Based on this, GRX EGFP‐Cav1 represents a new cellular model that can be an important tool for understanding of events related to HSC activation. Furthermore, our results reinforce the role of Cav‐1 as a molecular marker of HSC activation. Abstract : 1‐ Exogenous expression of caveolin‐1 (Cav‐1) leads hepatic stellate cells (HSC) to more activated phenotype 2‐ GRX EGFP‐Cav1 cell line is a first‐rate tool for liver fibrosis studying 3‐ Cav‐1 is a potential molecular marker for HSC activation.