Malassezia interaction with a reconstructed human epidermis: Keratinocyte immune response. Issue 10 (29th July 2019)

• Record Type: Journal Article
• Authors: Pedrosa, Ana F.; Lisboa, Carmen; Branco, Joana; Pellevoisin, Christian; Miranda, Isabel Marcos; Rodrigues, Acacio G.
• Is Part Of: Mycoses. Volume 62:Issue 10(2019)
• Page Start: 932
• Page End: 936
• Publication Date: 2019-07-29
• Subjects: antimicrobial peptides -- cytokines -- immune -- inflammation -- Malassezia furfur -- Malassezia sympodialis -- reconstructed human epidermis; Pathogenic fungi -- Periodicals; Medical mycology -- Periodicals; 616.969
• DOI: 10.1111/myc.12965
• Languages: English
• ISSNs: 0933-7407

Abstract

Summary: The immediate immune response developed by the keratinocytes against Malassezia yeasts has been addressed yielding conflicting results. This study aims the assessment of cytokines and antimicrobial peptides gene expression elicited by M. sympodialis and M. furfur once in contact with a reconstructed human epidermis. A yeast suspension was prepared in RPMI 1640 medium (Sigma‐Aldrich, St. Louis, MO) supplemented with Tween 60 and oleic acid to obtain approximately 1 × 10 6 cells in a volume of 100 μL. Clinical isolates of M. sympodialis (from pityriasis versicolor) and M. furfur (from seborrhoeic dermatitis) were inoculated, separately, onto a reconstructed human epidermis. A distinct expression pattern was found between the two tested species, with a tendency for overexpression of pro‐inflammatory cytokines very soon after infection, whereas no significant expression or gene downregulation was often noticed following 24 and 48 h of incubation. A possible Malassezia species‐dependent immune response pattern is highlighted.