Transformation and species identification of CuO nanoparticles in plant cells (Nicotiana tabacum). Issue 9 (5th August 2019)

• Record Type: Journal Article
• Authors: Dai, Yanhui; Zhao, Jian; Liu, Xiaoyun; Yu, Xiaoyu; Jiang, Zhixiang; Bu, Yuyu; Xu, Zefeng; Wang, Zhenyu; Zhu, Xiaoshan; Xing, Baoshan
• Is Part Of: Environmental science. Volume 6:Issue 9(2019)
• Page Start: 2724
• Page End: 2735
• Publication Date: 2019-08-05
• Subjects: Environmental sciences -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c9en00781d
• Languages: English
• ISSNs: 2051-8153

Abstract

Abstract : CuO NPs were transformed into Cu2 O, Cu2 S, and Cu acetate on cell walls and inside plant cells. Abstract : The transformation of CuO nanoparticles (NPs) during interaction with Nicotiana tabacum L. cv. bright yellow-2 (BY-2) cells was investigated in this study. CuO NPs were taken up, and transformed into Cu2 O, Cu2 S and Cu acetate after incubation with plant cells for 12 h based on high resolution transmission electron microscopy and X-ray absorption near-edge spectroscopy analysis. The transformation of CuO NPs initially occurred on cell walls with Cu2 O and Cu2 S being the main transformation products. Galacturonic acid as an important component of cell walls was mainly responsible for reducing CuO to Cu2 O and cysteine for the formation of Cu2 S. Cu 2+ ions could not be transformed into any Cu-based particles (CuO, Cu2 O, or Cu2 S) on cell walls. After internalization, CuO NPs could also be transformed into Cu2 O and Cu2 S through interacting with protoplasts as well as mitochondria, confirming that CuO NPs were able to transform intracellularly. Moreover, Cu2 O NPs showed higher toxicity to plant cells as compared to CuO NPs with similar individual sizes, indicating that the transformation of CuO NPs enhanced their toxicity to plant cells. These findings could provide new insights into better understanding the transformation and fate of metal-based NPs in living organisms and environments.