MicroRNA-145 promotes esophageal cancer cells proliferation and metastasis by targeting SMAD5. (3rd July 2018)

• Record Type: Journal Article
• Authors: Zhang, Qiong; Gan, Huaiyong; Song, Wenqing; Chai, Damin; Wu, Shiwu
• Is Part Of: Scandinavian journal of gastroenterology. Volume 53:Number 7(2018)
• Page Start: 769
• Page End: 776
• Publication Date: 2018-07-03
• Subjects: miR-145 -- SMAD5 -- esophageal cancer -- migration -- invasion; Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; 616.33
• DOI: 10.1080/00365521.2018.1476913
• Languages: English
• ISSNs: 0036-5521

Abstract

Abstract: Objective: To clarify the relative expression and molecular function of microRNA (miR)-145 in esophageal cancer and understand its mechanistic involvement in this disease. Material and methods: The relative expression of miR-145 in clinical samples was analyzed using the public GSE43732 dataset. The prognostic analysis with respect to miR-145 expression was performed with Kaplan–Meier plot. Cell viability was measured by MTT assay and the anchorage-independent growth was evaluated by soft agar assay. The migration and invasion of esophageal cancer cells were measured using transwell chamber. The regulatory effect of miR-145 on SMAD5 was determined by dual-luciferase reporter assay. The endogenous SMAD5 protein was measured by Western blot. Results: We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. Ectopic expression of miR-145 mimic significantly stimulated cell proliferation and anchorage-independent growth. Furthermore, high level of miR-145 significantly promoted both migration and invasion in vitro. Notably, we identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. Conclusion: Our study uncovered the crucial role of miR-145/SMAD5 in esophageal cancer and highlighted its target potential for diagnostic and therapeutic purpose.