A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection. Issue 1 (1st December 2016)

• Record Type: Journal Article
• Authors: Kashida, Hiromu; Morimoto, Kazuhiro; Asanuma, Hiroyuki
• Is Part Of: Science and technology of advanced materials. Volume 17:Issue 1(2016)
• Page Start: 267
• Page End: 273
• Publication Date: 2016-12-01
• Subjects: DNA -- Cy3 -- RNA imaging -- Fluorescence in situ hybridization; 30 Bio-inspired and biomedical materials -- 101 Self-assembly/Self-organized materials -- 208 Sensors and actuators -- 301 Chemical syntheses/processing -- 501 Chemical analyses -- 505 Optical/Molecular spectroscopy; Materials -- Technological innovations -- Periodicals; 620.112
• DOI: 10.1080/14686996.2016.1182412
• Languages: English
• ISSNs: 1468-6996

Abstract

Abstract: We herein report a stem-less probe for the detection of RNA that depends on pairing between Cy3 and nitro methyl red. In our design, two Cy3 residues and two nitro methyl red residues were introduced into an oligonucleotide. In the absence of the target, these dyes formed a complex, and emission of Cy3 was efficiently quenched. Hybridization with the target RNA disrupted this interaction and resulted in Cy3 emission. Under optimized conditions, the signal to background ratio was as high as 180. We demonstrated specific detection of target RNA in cells using a wash-free FISH protocol. Abstract :