Immune-modulation of two BATF3 paralogues in rainbow trout Oncorhynchus mykiss. (July 2018)

• Record Type: Journal Article
• Authors: Wang, Jun; Liu, Min; Wu, Yang; Yoon, Sohye; Alnabulsi, Abdo; Liu, Fuguo; Fernández-Álvarez, Clara; Wang, Tiehui; Holland, Jason W.; Secombes, Chris J.; Zou, Jun
• Is Part Of: Molecular immunology. Volume 99(2018:Jul.)
• Page Start: 104
• Page End: 114
• Publication Date: 2018-07
• Subjects: AP-1 activator protein 1 -- BATF3 basic leucine zipper transcription factor ATF-like (BATF) -3 -- bZIP basic leucine zipper -- CD cluster of differentiation -- DB DNA binding domain -- DC dendritic cell -- HK head kidney -- IFN interferon -- IL interleukin -- IRF interferon regulated factor -- LPS lipopolysaccharide -- LZ leucine zipper -- PAMP pathogen-associated molecular pattern -- PHA phytohaemagglutinin -- polyI:C polyinosinic:polycytidylic acid -- TLR Toll-like receptor; BATF3 -- Transcription factor -- Leucocyte differentiation -- Bacterial and viral infection -- Rainbow trout; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2018.04.016
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: The BATF3 genes were analysed in detail for the first time in fish. Trout BATF3a is highly expressed in spleen, liver and pyloric caeca. Trout BATF3a is highly up-regulated in monocytes/macrophages by IFNγ treatment. Both BATF3a and BATF3b are induced after infection with bacterial and viral diseases. Abstract: Basic leucine zipper transcription factor ATF-like (BATF) -3 is a member of the activator protein 1 (AP‑1) family of transcription factors and is known to play a vital role in regulating differentiation of antigen-presenting cells in mammals. In this study, two BATF3 homologues (termed BATF3a and BATF3b) have been identified in rainbow trout ( Oncorhynchus mykiss ). Both genes were constitutively expressed in tissues, with particularly high levels of BATF3a in spleen, liver, pyloric caecae and head kidney. BATF3a was also more highly induced by PAMPs and cytokines in cultured cells, with type II IFN a particularly potent inducer. In rIL-4/13 pre-stimulated cells, the viral PAMPS polyI:C and R848 had the most pronounced effect on BATF3 expression. BATF3 expression could also be modulated in vivo, following infection with Yersinia ruckeri, a bacterial pathogen causing redmouth disease in salmonids, or with the rhabdovirus IHNV. The results suggest that BATF3 may be functionally conserved in regulating the differentiation and activation of immune cells in lower vertebrates and could be explored as a potential marker for comparative investigation of leucocyte lineage commitment across the vertebrate phyla.