Effects of anoxia on survival and gene expression in Bactrocera dorsalis. (May 2018)

• Record Type: Journal Article
• Authors: Deng, Yufang; Hu, Fan; Ren, Lili; Gao, Xiwu; Wang, Yuejin
• Is Part Of: Journal of insect physiology. Volume 107(2018)
• Page Start: 186
• Page End: 196
• Publication Date: 2018-05
• Subjects: HIF hypoxia inducible factor -- bHLH basic helix-loop-helix -- PAS Per-Arnt-Sim -- Hsp heat shock protein -- SOD superoxide dismutase -- HIF-1 hypoxia inducible factor 1 -- VHL von Hippel–Lindau -- HRE hypoxia response element -- CAT catalase -- POX peroxidase -- GST glutathione S-transferases -- RACE rapid amplification of cDNA ends -- ORF open reading frame -- 3′UTR 3′-untranslated region -- 5′UTR 5′-untranslated region -- IRES Internal Ribosome Entry Site -- Hsf heat shock factor; Anoxia -- Hypoxia inducible factor 1α -- Hypoxia inducible factor 1β -- Heat shock protein -- Superoxide dismutase; Insects -- Physiology -- Periodicals; Insectes -- Physiologie -- Périodiques; Insects -- Physiology; Periodicals; 571.157
• DOI: 10.1016/j.jinsphys.2018.04.004
• Languages: English
• ISSNs: 0022-1910

Abstract

Graphical abstract: Highlights: The B. dorsalis larvae were quite tolerant to anoxia conditions. HIF-1α and HIF-1β were firstly cloned and characterized in B. dorsalis . HIF-1α can be induced by hypoxia, whereas HIF-1β expression was not changed. Three major Hsps expression increased significantly during anoxia and recovery. B. dorsalis has a strategy to survive in the low oxygen condition. Abstract: The oriental fruit fly ( Bactrocera dorsalis ) larvae may commonly experience a hypoxia microenvironment and have evolved the ability to survive in the low oxygen condition with some physiological and biochemical mechanisms. However, little is known about the response of B. dorsalis to hypoxia or anoxia. In this study, the effect of anoxia on the survival of B. dorsalis was investigated . The results showed that the B. dorsalis larvae were quite tolerant to anoxia conditions and can tolerate up to 24 h of anoxia exposure without a significant reduction in survival, 100% mortality was reached after 84 h of anoxia exposure. The cDNA of hypoxia inducible factor (HIF) 1α and HIF-1β is 2912 and 3618 bp in length, encoding 766 and 648 amino acid residues, respectively. Both HIF-1α and HIF-1β contain conserved basic helix-loop-helix (bHLH) domain and Per-Arnt-Sim (PAS) domain. HIF-1α can be induced by hypoxia, whereas HIF-1β expression was not significantly changed with the oxygen concentration. Three major heat shock proteins (Hsps) expression increased significantly during anoxia and recovery and Hsp70 was the most responsive to anoxia. Four superoxide dismutase (SOD) genes expression were also up-regulated during anoxia exposure. These data suggest that B. dorsalis has a strategy to induce HIF-1α and HIF-1-responsive genes to survive in the low oxygen condition.