Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster. (May 2018)

• Record Type: Journal Article
• Authors: Ibrahim, Emad; Dobeš, Pavel; Kunc, Martin; Hyršl, Pavel; Kodrík, Dalibor
• Is Part Of: Journal of insect physiology. Volume 107(2018)
• Page Start: 167
• Page End: 174
• Publication Date: 2018-05
• Subjects: Adipokinetic hormone -- Adenosine -- Drosophila -- Nematode -- Oxidative stress -- Locomotion; Insects -- Physiology -- Periodicals; Insectes -- Physiologie -- Périodiques; Insects -- Physiology; Periodicals; 571.157
• DOI: 10.1016/j.jinsphys.2018.04.002
• Languages: English
• ISSNs: 0022-1910

Abstract

Graphical abstract: Highlights: AKH increases Drosophila mortality elicited by entomopathogenic nematodes. Nematode infection modulates level of nutrients in haemolymph. Nematode infection increases total antioxidant capacity. AKH application affects Drosophila locomotion. Abstract: This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora ). Three loss-of-function mutant larvae were tested: Akh 1, AdoR 1 (adenosine receptor), and Akh 1 AdoR 1 . Mortality decreased in all mutants post-EPN infection compared with the control ( w 1118 ). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w 1118 and Akh 1 larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR 1 and Akh 1 AdoR 1 mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh 1 . In uninfected larvae mutations decreased antioxidative capacity in Akh 1 and increased in AdoR 1, however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w 1118 larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.