Deoxynivalenol-induced oxidative stress and Nrf2 translocation in maternal liver on gestation day 12.5 d and 18.5 d. (1st April 2019)

• Record Type: Journal Article
• Authors: Yu, Miao; Peng, Zhao; Liao, Yuxiao; Wang, Liangliang; Li, Dan; Qin, Chenyuan; Hu, Jiawei; Wang, Zhenting; Cai, Mengyao; Cai, Qiang; Zhou, Feng; Shi, Shaojun; Yang, Wei
• Is Part Of: Toxicon. Volume 161(2019)
• Page Start: 17
• Page End: 22
• Publication Date: 2019-04-01
• Subjects: Deoxynivalenol (DON) -- Hepatotoxicity -- Oxidative stress -- HO-1 -- Nrf2 translocation; Alanine aminotransferase ALT -- Antioxidant response element ARE -- Aspertate aminotransferase AST -- Catalase CAT -- Deoxynivalenol DON -- DON high dose group DON-H -- DON low dose group DON-L -- Gestation day GD -- Glutathione GSH -- Glutathione peroxidase GPx -- Hematoxylin and eosin H&E -- Heme oxygenase-1 HO-1 -- Kelch-like ECH-associated protein 1 Keap1 -- Malondialdehyde MDA -- Mean fluorescence intensity MFI -- NF-E2-related factor 2 Nrf2 -- Reactive oxygen species ROS -- Specific Pathogen Free SPF -- Superoxide Dismutase SOD; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2019.02.018
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Deoxynivalenol (DON) contamination is indicated as a worldwide problem since it causes economic losses for the grain and is a potential threat to both animal and human health. This study concentrated on DON-induced oxidative damage and the accompanying nuclear factor erythroid 2-related factor 2 (Nrf2) translocation during DON-induced maternal hepatotoxicity. The 0, 1.0 and 2.5 mg/kg/day of DON were used as doses for the experiment during gestation days. DON slightly increased the levels of ALT and AST in GD12.5 d instead of GD18.5 d. Oxidative stress and anti-oxidization system were both found to be activated in the experiment which marked by ROS, MDA and GSH increasing, especially on GD12.5 d. The levels of HO-1 were significantly increased by DON exposure at different two time points. Moreover, Nrf2 translocation appeared both in GD 12.5 d and GD 18.5 d. In conclusion, DON-induced ROS accumulation may cause maternal liver damage in the initial stages, but the related stimulation of Nrf2/HO-1 pathway improves the removal of ROS and decreases the level of oxidative stress thereby protecting the liver damage. Therefore, upregulating the Nrf2-dependent response is one of the potential methods that protects maternal liver from DON-induced oxidative damage. Graphical abstract: Image 1 Highlights: DON-induced ROS accumulation may cause liver damage of pregnant mice. Nrf2/HO-1 pathway improves the removal of ROS and decreases oxidative stress. Nrf2/HO-1 pathway protects liver from DON-induced oxidative damage.