Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves. (January 2019)

• Record Type: Journal Article
• Authors: Saggaf, Maliha H.; Ndunguru, Joseph; Tairo, Fred; Sseruwagi, Peter; Ascencio-Ibáñez, José Trino; Kilalo, Dora; Miano, Douglas W.
• Is Part Of: Physiological and molecular plant pathology. Volume 105(2019)
• Page Start: 67
• Page End: 76
• Publication Date: 2019-01
• Subjects: Manihot esculenta -- Cassava brown streak virus (CBSV) -- Immunohistochemistry -- Cassava brown streak disease -- Immunolocalization; Plant diseases -- Periodicals; Diseased plants -- Physiology -- Periodicals; Phytopathogenic microorganisms -- Host plants -- Periodicals; 632
• DOI: 10.1016/j.pmpp.2018.06.001
• Languages: English
• ISSNs: 0885-5765

Abstract

Abstract: The localization of Cassava brown streak virus (CBSV) in cassava ( Manihot esculenta ) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger ( P < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava. Highlights: Immunohistochemistry can be used to localize CBSV within infected leaf tissues. CBSV is frequent in abaxial and adaxial epidermal cells but rare in the phloem. CBSV infection influences the cellular morphology of the infected leaf tissues.