A Carbapenem‐Based Off–On Fluorescent Probe for Specific Detection of Metallo‐β‐Lactamase Activities. (10th June 2018)

• Record Type: Journal Article
• Authors: Mao, Wuyu; Wang, Yaqun; Qian, Xiana; Xia, Lingying; Xie, Hexin
• Is Part Of: Chembiochem. Volume 20:Number 4(2019)
• Page Start: 511
• Page End: 515
• Publication Date: 2018-06-10
• Subjects: antibiotics -- fluorescent probes -- lactams -- metalloenzymes -- sensors; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201800126
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Metallo‐β‐lactamase is one of the major clinical threats because this β‐lactam‐hydrolyzing enzyme confers significant resistance to most β‐lactam antibiotics, including carbapenems, among bacterial pathogens. Reported herein is a novel fluorogenic sensor for the specific detection of metallo‐β‐lactamase activities. This carbapenem‐based reagent exhibits excellent selectivity to metallo‐β‐lactamase over other serine‐β‐lactamases, including serine carbapenemases. The usefulness of this probe was further demonstrated in the rapid identification of metallo‐β‐lactamase‐expressing pathogenic bacteria. Abstract : Overcoming resistance : A new fluorogenic sensor for the detection of metallo‐β‐lactamase activities has been disclosed. With carbapenem as an enzymatic recognition moiety, this reagent shows excellent specificity to metallo‐β‐lactamase over other serine‐β‐lactamases, including serine carbapenemases, and allows the rapid identification of metallo‐β‐lactamase‐expressing pathogenic bacteria with high selectivity.