A novel collagen glycopeptide, Pro-Hyp-CONH-GlcN, stimulates cell proliferation and hyaluronan production in cultured human dermal fibroblasts. (June 2018)

• Record Type: Journal Article
• Authors: Feng, Mengmeng; Betti, Mirko
• Is Part Of: Journal of functional foods. Volume 45(2018)
• Page Start: 277
• Page End: 287
• Publication Date: 2018-06
• Subjects: POGlcN Pro-Hyp-CONH-GlcN -- PO Pro-Hyp -- GlcN glucosamine -- HDF human dermal fibroblast -- ECM extracellular matrix -- HA hyaluronic acid -- CH collagen hydrolysates -- qRT-PCR quantitative reverse transcription polymerase chain reaction -- UHPLC ultra-high pressure liquid chromatography -- ANOVA analysis of variance -- M phase mitosis phase -- DAPI 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride -- BSA bovine serum albumin -- bFGF fibroblast growth factor -- Edu 5-Ethynyl-2′-deoxyuridine -- PBS phosphate buffered saline -- DMEM Dulbecco's Modified Eagle Medium -- FBS fetal bovine serum -- HEPES N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Collagen glycopeptide -- Pro-Hyp-CONH-GlcN -- Dermal fibroblast -- Pro-Hyp -- Hyaluronan; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.04.022
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Collagen-derived glycopeptide Pro-Hyp-CONH-GlcN stimulated skin fibroblasts' growth. The glycopeptide improved the hyaluronan biosynthesis by skin fibroblasts. The glycopeptide arrested more fibroblasts that are active in DNA synthesis phase. The glycopeptide up-regulated the expression of hyaluronan synthase-2 and -3 gene. Abstract: Pro-Hyp-CONH-GlcN (POGlcN) is a glycopeptide synthesized from a typical bioavailable collagen peptide Pro-Hyp by conjugation with glucosamine. This study investigated the effects of POGlcN on dermal fibroblast growth, hyaluronan production, and the underlying mechanism. The results suggest that the plasma digestion-resistant POGlcN stimulated the proliferation of cultured human dermal fibroblasts in a time- and concentration-dependent manner with optimal performance at 200 nmol/mL and 6 days. Comparable to Pro-Hyp, POGlcN increased the cell number to 1.5-fold and HA production to 1.4-fold of control. Edu labeling-assisted cell cycle analysis revealed that POGlcN modulated the cell cycle of fibroblasts by arresting more cells in DNA synthesis phase. Moreover, POGlcN up-regulated both hyaluronan synthase-2 and -3 gene transcriptions to 1.8-fold and 2.0-fold of control, respectively. The results implied the potency of POGlcN for improving skin health and skin regeneration.