Medium optimization for production of anti-streptococcal liamocins by Aureobasidium pullulans. (January 2018)

• Record Type: Journal Article
• Authors: Leathers, Timothy D.; Skory, Christopher D.; Price, Neil P.J.; Nunnally, Melinda S.
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 13(2018)
• Page Start: 53
• Page End: 57
• Publication Date: 2018-01
• Subjects: Anti-Streptococcal -- Aureobasidium pullulans -- Liamocins -- Medium optimization; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2017.11.008
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: Liamocins are antimicrobial compounds with specificity for Streptococcus spp., produced by certain strains of the fungus Aureobasidium pullulans . Recent studies have identified strains and culture medium for liamocin production. However, this culture medium has not previously been optimized for liamocin production. In this study, we employ one-factor-at-a-time and statistical methods to develop an optimized medium for liamocin production. Liamocin yields from optimized medium were nearly double those from standard medium, up to 22 g liamocin/L. This represents the highest yields of liamocin reported to date. Furthermore, MALDI-TOF analyses show that liamocins produced from optimized medium are similar to those produced from standard medium. Graphical abstract: fx1 Highlights: Culture medium was optimized for production of anti-streptococcal liamocins. Yields from optimized medium were nearly double those from standard medium, up to 22 g liamocin/L. These are the highest yields of liamocin reported to date. Liamocins produced from optimized medium are similar to those produced from standard medium.