A novel enzyme catalysis reactor based on superparamagnetic nanoparticles for biotechnological applications. Issue 5 (October 2018)

• Record Type: Journal Article
• Authors: Moldes-Diz, Yolanda; Eibes, Gemma; Vázquez-Vázquez, Carlos; Fondado, Alfonso; Mira, Jorge; Feijoo, Gumersindo; Lema, Juan M.; Moreira, Maria Teresa
• Is Part Of: Journal of environmental chemical engineering. Volume 6:Issue 5(2018)
• Page Start: 5950
• Page End: 5960
• Publication Date: 2018-10
• Subjects: Enzymatic bioreactor -- Magnetic nanoparticle -- Methyl green -- Internal magnetic separator -- Laccase; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2018.09.014
• Languages: English
• ISSNs: 2213-2929

Abstract

Graphical abstract: Highlights: A magnetic separation unit of toroidal magnets in alternate polarity was designed. Laccase immobilized on magnetic nanoparticles was successfully recovered. Methyl green and rutin were successfully transformed in the magnetic enzymatic reactor. The scalability and economic feasibility of the process were investigated. Abstract: In this research, a new sequential batch reactor (SBR) coupled to an internal magnetic separator was developed. The separator consists of a set of axially magnetized permanent toroidal magnets, distributed along a non-magnetic steel rod, uniformly spaced with alternate polarity, which provide an external magnetic field of up to 1.2 T. The feasibility of magnetic separation was assessed for the retention of the nanobiocatalyst based on laccase immobilized on silica-coated MNPs. The proof of concept was evaluated for the enzymatic decolorization of the methyl green (MG) dye and the oligomerization of rutin, with virtually complete recovery of the nanobiocatalyst (99%) and high biotransformation efficiency of both compunds. The characterization of the reaction products of MG by laccase was conducted by high performance liquid chromatography (HPLC) with a diode array detector (DAD) coupled to mass spectrometry (MS) using the electrospray ionization source (ESI) (HPLC-DAD-ESI-MS). Moreover, the biotransformation products showed less microtoxicity than the parent compound and increased biodegradability. Beyond laboratory scale experiments, the reactor proposed here was scaled-up to a volume of 100 L and the environmental performance and cost analysis were estimated.