Effects of microvirin monomers and oligomers on hepatitis C virus. Issue 3 (30th June 2017)

• Record Type: Journal Article
• Authors: Min, Yuan-Qin; Duan, Xu-Chu; Zhou, Yi-Dan; Kulinich, Anna; Meng, Wang; Cai, Zhi-Peng; Ma, Hong-Yu; Liu, Li; Zhang, Xiao-Lian; Voglmeir, Josef
• Is Part Of: Bioscience reports. Volume 37:Issue 3(2017)
• Publication Date: 2017-06-30
• Subjects: high-mannose glycan -- hepatitis C virus -- lectin oligomer -- Microvirin; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1042/BSR20170015
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Microvirin (MVN) is a carbohydrate-binding protein which shows high specificity for high-mannose type N-glycan structures. In the present study, we tried to identify whether MVN could bind to high-mannose containing hepatitis C virus (HCV) envelope glycoproteins, which are heavily decorated high-mannose glycans. In addition, recombinantly expressed MVN oligomers in di-, tri- and tetrameric form were evaluated for their viral inhibition. MVN oligomers bound more efficiently to HCV virions, and displayed in comparison with the MVN monomer a higher neutralization potency against HCV infection. The antiviral effect was furthermore affected by the peptide linker sequence connecting the MVN monomers. The results indicate that MVN oligomers such as trimers and tetramers may be used as future neutralization agents against HCV infections.