Evaluation of the biocompatibility of resin composite‐based dental materials with gingival mesenchymal stromal cells. Issue 10 (16th July 2019)

• Record Type: Journal Article
• Authors: Cândea Ciurea, Andreea; Şurlin, Petra; Stratul, Ştefan‐Ioan; Soancă, Andrada; Roman, Alexandra; Moldovan, Mărioara; Tudoran B., Lucian; Pall, Emoke
• Is Part Of: Microscopy research and technique. Volume 82:Issue 10(2019)
• Page Start: 1768
• Page End: 1778
• Publication Date: 2019-07-16
• Subjects: biocompatibility -- gingiva -- ormocer -- resin composite -- stem cells; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23343
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Resin composite‐based dental materials can leach certain components into the oral environment, causing potentially harmful gingival biological effect. Gingival tissue is a rich source of mesenchymal stem cells (MSCs) that is easily accessible, and can be used as a complementary approach for the investigation of dental material biocompatibility. Using gingival MSCs (gMSCs), the present study aimed to investigate the cytotoxicity of two classes of restorative dental materials (ormocers and resin composites) used to restore class II cavities close to the gingival margin, in addition to analyzing the leached compounds from these resin composite‐based materials. Functionality assays (Colony‐forming unit, migratory potential, and proliferation assays) and a viability assay (MTT) were employed. Cells' aspect was observed by scanning electron microscopy (SEM). Leached monomers were also quantitated using high‐performance liquid chromatography (HPLC). The cytotoxicity of the biomaterials was highlighted by impaired functionality and diminished viability of gMSCs. Despite being variants of the same commercial material, the two ormocers behaved differently one material having a more negative impact on cell functionality than the other. Cells appeared to attach well to all materials. Main monomer molecules were mostly released by the tested materials. For all samples, an increased elution of monomers was recorded in artificial saliva as compared with culture medium. One composite material has released nearly eight times more urethane dimetacrylate in artificial saliva than in culture medium. Significantly lower gMSC viability scores were recorded for all the investigated samples in comparison with the control. Abstract : Gingival mesenchymal stem cells present numerous cytoplasmic processes that appear to maintain attachment to the dental restorative ormocer material. All tested dental materials induced a cytotoxic effect as highlighted by impaired functionality and diminished viability of gMSCs. The major monomer molecules released by the tested materials were BisGMA and UDMA.