Novel microfluidic device integrated with a fluidic‐capacitor to mimic heart beating for generation of functional liver organoids. Issue 10 (19th August 2019)

• Record Type: Journal Article
• Authors: Wu, Jiaxu; Hirai, Yoshikazu; Kamei, Ken‐Ichiro; Tsuchiya, Toshiyuki; Tabata, Osamu
• Is Part Of: Electronics and communications in Japan. Volume 102:Issue 10(2019)
• Page Start: 41
• Page End: 49
• Publication Date: 2019-08-19
• Subjects: equivalent circuit model -- fluidic capacitor -- organoid -- polydimethylsiloxane -- pressure stimulus; Electronics -- Periodicals; Telecommunication -- Periodicals; 621.6805
• DOI: 10.1002/ecj.12210
• Languages: English
• ISSNs: 1942-9533

Abstract

Abstract: Liver organoids hold a great potential to understand liver development and contribute for drug screening and toxicological testing. However, conventional methods to generate organoids provide insufficient liver functions and less reproducibility, due to lack of controllability of cellular microenvironmental cues. To tackle these issues, we focus on one of the environmental cues, pressure stimuli by heart beating, and develop a microfluidic‐based cell culture platform integrating a fluidic capacitor to produce pressure stimuli with hydrostatic pressure. Furthermore, we demonstrate a numerical simulation based on equivalent circuit model for designing the device parameters with sufficient accuracy. This device concept will provide insights into physical micro‐environmental regulation on organoid development.