Identification of a crucial amino acid implicated in the hydroxylation/desaturation ratio of CpFAH12 bifunctional hydroxylase. Issue 10 (21st July 2019)

• Record Type: Journal Article
• Authors: Robin, Julien; Gueroult, Marc; Cheikhrouhou, Randa; Guicherd, Marie; Borsenberger, Vinciane; Marty, Alain; Bordes, Florence
• Is Part Of: Biotechnology and bioengineering. Volume 116:Issue 10(2019)
• Page Start: 2451
• Page End: 2462
• Publication Date: 2019-07-21
• Subjects: hydroxylation‐desaturation specificity -- membrane desaturases -- modeling -- mutagenesis and chimeras -- Yarrowia lipolytica; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.27102
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: Claviceps purpurea bifunctional Δ12‐hydroxylase/desaturase, CpFAH12, and monofunctional desaturase CpFAD2, share 86% of sequence identity. To identify the underlying determinants of the hydroxylation/desaturation specificity, chimeras of these two enzymes were tested for their fatty acid production in an engineered Yarrowia lipolytica strain. It reveals that transmembrane helices are not involved in the hydroxylation/desaturation specificity whereas all cytosolic domains have an impact on it. Especially, replacing the CpFAH12 cytosolic part near the second histidine‐box by the corresponding CpFAD2 part annihilates all hydroxylation activity. Further mutagenesis experiments within this domain identified isoleucine 198 as the crucial element for the hydroxylation activity of CpFAH12. Monofunctional variants performing the only desaturation were obtained when this position was exchanged by the threonine of CpFAD2. Saturation mutagenesis at this position showed modulation in the hydroxylation/desaturation specificity in the different variants. The WT enzyme was demonstrated as the most efficient for ricinoleic acid production and some variants showed a better desaturation activity. A model based on the recently discovered membrane desaturase structures indicate that these changes in specificity are more likely due to modifications in the di‐iron center geometry rather than changes in the substrate binding mode. Abstract : Few enzymes are known to perform the fatty acid hydroxylation leading to ricinoleic acid, a product of industrial interest. The bifunctional CpFAH12 hydroxylase is one of them. We identified isoleucine 198 as a crucial position for the desaturation/hydroxylation specificity in this enzyme.