Facile synthesis of (−)‐vibo‐quercitol from maltodextrin via an in vitro synthetic enzymatic biosystem. Issue 10 (12th July 2019)

• Record Type: Journal Article
• Authors: Bai, Xue; Meng, Dongdong; Wei, Xinlei; Zhou, Xigui; Lu, Fuping; You, Chun
• Is Part Of: Biotechnology and bioengineering. Volume 116:Issue 10(2019)
• Page Start: 2710
• Page End: 2719
• Publication Date: 2019-07-12
• Subjects: (−)‐vibo‐quercitol -- cascade biocatalysis -- in vitro synthetic enzymatic biosystem -- maltodextrin; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.27096
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: (−)‐ vibo ‐Quercitol (VQ: 1L‐1, 2, 4/3, 5‐cyclohexanepentol), a form of deoxyinositol, is an alternative chiral building block in the synthesis of bioactive compounds to control diabetes. In this study, an adenosine triphosphate‐free in vitro synthetic enzymatic biosystem composed of five enzymes (including one enzyme for NADH regeneration) was constructed to produce VQ from maltodextrin in one‐pot. After optimization of reaction conditions, 7.6 g/L VQ was produced from 10 g/L maltodextrin with a product yield (mol/mol) of 77%, and 25.3 g/L VQ with a purity of 87% was produced from 50 g/L maltodextrin through simple scaling up of this nonfermentative enzymatic biosystem. Therefore, this study provides an economical and environmentally friendly method for the envisioned quercitol biosynthesis. Abstract : (−)‐vibo‐Quercitol is a potential intermediate for manufacturing pharmaceuticals to diabetes. Here, Bai and coworkers successfully demonstrated a novel adenosine triphosphate‐free in vitro synthetic enzymatic biosystem composed of five enzymes to produce (−)‐vibo‐quercitol from inexpensive maltodextrin in one vessel. This study provides a cost‐effective approach for chiral quercitols biosynthesis.