Editing streptomycete genomes in the CRISPR/Cas9 age. Issue 9 (25th January 2019)

• Record Type: Journal Article
• Authors: Alberti, Fabrizio; Corre, Christophe
• Is Part Of: Natural product reports. Volume 36:Issue 9(2019)
• Page Start: 1237
• Page End: 1248
• Publication Date: 2019-01-25
• Subjects: Natural products -- Periodicals; Produits naturels -- Périodiques; 547.7
• DOI: 10.1039/c8np00081f
• Languages: English
• ISSNs: 0265-0568

Abstract

Abstract : This article reviews CRISPR/Cas9-based toolkits available to investigate natural product biosynthesis and regulation in streptomycete bacteria. Abstract : Covering: up to December 2018 This article aims to highlight advantages, drawbacks and issues that users should consider when implementing the use of CRISPR/Cas9-tools for genome editing in streptomycetes, the most prolific source of antimicrobial natural products to date. Here, we examine four toolkits that have so far been made available for streptomycete in vivo -engineering and one for in vitro -editing, and review how they have been applied over the last three years. Our critical evaluation of these toolkits intends to support potential users in determining what they could achieve, what they should consider and what system they should select/optimise for their application.