Dual-quenching NBD-based fluorescent probes for separate detection of H2S and Cys/Hcy in living cells. Issue 36 (29th August 2019)

• Record Type: Journal Article
• Authors: Jiang, Yaqing; Ji, Xiuru; Zhang, Changyu; Xi, Zhen; Sun, Lu; Yi, Long
• Is Part Of: Organic & biomolecular chemistry. Volume 17:Issue 36(2019)
• Page Start: 8435
• Page End: 8442
• Publication Date: 2019-08-29
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c9ob01535c
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Dual-quenching fluorescent probes based on thiolysis of NBD thioether/ether/amine for fast and separate detection of H2 S and Cys/Hcy in living cells were rationally constructed. Abstract : Biothiols are implicated in different physiological and pathological processes, while abnormal levels of biothiols are related to numerous diseases. It is essential to discriminatively detect these molecules and explore their inherent transformation in living biological samples. Herein, dual-reactive and dual-quenching fluorescent probes based on thiolysis of NBD (7-nitro-1, 2, 3-benzoxadiazole) thioether/ether/amine for separate detection of H2 S and Cys/Hcy were rationally designed. Probe 2 was able to sense H2 S ( λ ex/em = 405/455 nm) and Cys/Hcy ( λ ex/em = 470/550 nm) in the presence of GSH in aqueous buffer. Moreover, the probe not only could be applied for visualization of exogenous H2 S and Cys in living cells but also successfully showcased the potential to verify the biosynthesis of endogenous H2 S from Cys.