Mechanistic insight into the roles of Pseudomonas plecoglossicida clpV gene in host-pathogen interactions with Larimichthys crocea by dual RNA-seq. Issue 93 (October 2019)

• Record Type: Journal Article
• Authors: Tang, Yi; Sun, Yujia; Zhao, Lingmin; Xu, Xiaojin; Huang, Lixing; Qin, Yingxue; Su, Yongquan; Yi, Ganfeng; Yan, Qingpi
• Is Part Of: Fish & shellfish immunology. Issue 93(2019)
• Page Start: 344
• Page End: 353
• Publication Date: 2019-10
• Subjects: Host-pathogen interaction -- Larimichthys crocea -- Pseudomonas plecoglossicida -- clpV -- Dual RNA-seq; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.07.066
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Large yellow croaker ( Larimichthys crocea ) is an economical important farmed fish in China. "Visceral White Spot Disease" caused by Pseudomonas plecoglossicida is a disease with a high mortality rate in cage-cultured L. crocea in recent years and resulted in heavy economy lossess. The dual RNA-seq results of previous study showed that the expression of clpV gene in P. plecoglossicida was significantly up-regulated during infection. RNAi significantly reduced the expression of clpV in P. plecoglossicida with maximum silencing efficiency of 96.1%. Compared with the wild type strain, infection of clpV -RNAi strain resulted in a delayed onset time and a 25% reduction in mortality of L. crocea, as well as lessening the symptoms of the spleen. The results of dual RNA-seq of L. crocea infected by clpV -RNAi strain of P. plecoglossicida changed considerably, compared with the counterpart infected with the wild strain. The KEGG enrichment analysis showed that Cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway, C-type lectin receptor signaling pathway and MAPK signaling pathway of L. crocea were most affected by the silence of clpV in P. plecoglossicida . RNAi of clpV resulted in the downregulation of genes in flagella assembly pathway and a weaker immune response of host. Highlights: RNAi significantly reduced the expression of Pseudomonas plecoglossicida clpV gene. Dual RNA-seq simultaneously monitored host and pathogen transcriptome in a sample. The immune response of large yellow croaker to the infection clpV -RNAi strain was weaker. RNAi of clpV down-regulated the expression of flagella assembly pathway genes.