Involvement of apoptosis in the dialogue between the parasite Bonamia ostreae and the flat oyster Ostrea edulis. Issue 93 (October 2019)

• Record Type: Journal Article
• Authors: Gervais, Ophélie; Chollet, Bruno; Dubreuil, Christine; Durante, Serena; Feng, Chunyan; Hénard, Cyril; Lecadet, Cyrielle; Serpin, Delphine; Tristan, Renault; Arzul, Isabelle
• Is Part Of: Fish & shellfish immunology. Issue 93(2019)
• Page Start: 958
• Page End: 964
• Publication Date: 2019-10
• Subjects: Apoptosis -- Ostrea edulis -- Bonamia ostreae -- Flow cytometry -- Gene expression -- Mollusk; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.08.035
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The protozoan parasite Bonamia ostreae has been associated with the decline of flat oyster Ostrea edulis populations in some European countries. Control of shellfish diseases mostly relies on prevention measures including transfer restrictions and stock management measures such as breeding programmes. These prevention and mitigation measures require a better understanding of interactions between host and pathogens. Previous in vitro studies allowed identifying apoptosis as a mechanism activated by the flat oyster in response to B. ostreae . However, these experiments also suggested that the parasite is able to regulate apoptosis in order to survive and multiply within hemocytes. By simplifying the conditions of infection, in vitro studies allow identifying most distinct features of the response of the host. In order to appreciate the relative importance of apoptosis in this response at the oyster scale, in vivo trials were carried out by injecting with parasites oysters from two French locations, Quiberon Bay (Brittany) and Diana Lagoon (Corsica). Apoptosis was investigated on pools of hemolymph from oysters collected at early and later times after injection using previously developed tools. Apoptotic cellular activities including intracytoplasmic calcium concentration, mitochondrial membrane potential and phosphatidyl serine externalization were analysed using flow cytometry. Moreover, the expression of flat oyster genes involved in both extrinsic and intrinsic pathways was measured using real time quantitative PCR. Graphical abstract: Image 1 Highlights: In vivo infection with B. ostreae induces apoptotic response in flat oyster. Oyster apoptotic response evolves depending on infection status. Oyster origin influences the apoptotic response against B. ostreae .