Probiotic expressing heterologous phytase improves the immune system and attenuates inflammatory response in zebrafish fed with a diet rich in soybean meal. Issue 93 (October 2019)

• Record Type: Journal Article
• Authors: Santos, Kamila Oliveira; Costa-Filho, João; Riet, Jade; Spagnol, Kérolin Luana; Nornberg, Bruna Félix; Kütter, Mateus Tavares; Tesser, Marcelo Borges; Marins, Luis Fernando
• Is Part Of: Fish & shellfish immunology. Issue 93(2019)
• Page Start: 652
• Page End: 658
• Publication Date: 2019-10
• Subjects: Phytate -- Transgenic probiotic -- Condition factor -- Immune system -- Inflammation response -- Oxidative stress; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.08.030
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Although aquaculture is among the fastest growing food production sectors in the world, one of the bottlenecks for the continuity of its expansion is the dependence of animal protein on commercial feed formulations. Vegetable proteins are an alternative due to the low cost and high availability. However, this protein source is accompanied by a series of antinutritional and pro-inflammatory compounds, including phytate. Phytases can be added in feed for phytate degradation and increase nutrient availability. However, the use of purified phytases significantly increases the production costs. An interesting alternative is to use probiotics genetically modified as bioreactors for phytase production. In the present study, a strain of Bacillus subtilis secreting a fungal phytase was used to evaluate the effect of a feed with high content of soybean meal on zebrafish ( Danio rerio ). We analysed the condition factor (K) of fish, and the expression of genes related to the immune system, inflammatory response and oxidative. stress. The results obtained demonstrate that the transgenic probiotic was efficient in improving the fish condition factor, stimulating the immune system, reducing the inflammatory response and oxidative stress. Thus, probiotics acting as phytase bioreactors can be considered an interesting tool for the adaptation of commercial species to feed of lower cost. Highlights: Vegetable proteins are a low cost alternative for commercial feed used in aquaculture. Phytate is an antinutritional compound associated to vegetable matter. Phytases can degrade phytate and increase nutrient availability. Bacillus subtilis can be manipulated to secrete recombinant phytase.