Evaluation of transcutaneous immunization as a delivery route for an enterotoxigenic E. coli adhesin-based vaccine with CfaE, the colonization factor antigen 1 (CFA/I) tip adhesin. Issue 42 (30th September 2019)

• Record Type: Journal Article
• Authors: Rollenhagen, Julianne E.; Woods, Colleen M.; O'Dowd, Aisling; Poole, Steven T.; Tian, Jing-Hui; Guebre-Xabier, Mimi; Ellingsworth, Larry; Prouty, Michael G.; Glenn, Greg; Savarino, Stephen J.
• Is Part Of: Vaccine. Volume 37:Issue 42(2019)
• Page Start: 6134
• Page End: 6138
• Publication Date: 2019-09-30
• Subjects: ETEC -- TCI -- CFA/I -- CfaE -- Transcutaneous -- Adhesin; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2019.08.057
• Languages: English
• ISSNs: 0264-410X

Abstract

Abstract: dscCfaE is a recombinant form of the CFA/I tip adhesin CfaE, expressed by a large proportion of enterotoxigenic E. coli (ETEC). It is highly immunogenic by the intranasal route in mice and Aotus nancymaae, protective against challenge with CFA/I+ ETEC in an A. nancymaae challenge model, and antibodies to dscCfaE passively protect against CFA/I+ ETEC challenge in human volunteers. Here, we show that transcutaneous immunization (TCI) with dscCfaE in mice resulted in strong anti-CfaE IgG serum responses, with a clear dose-response effect. Co-administration with heat-labile enterotoxin (LT) resulted in enhanced immune responses over those elicited by dscCfaE alone and strong anti-LT antibody responses. The highest dose of dscCfaE administered transcutaneously with LT elicited strong HAI titers, a surrogate for the neutralization of intestinal adhesion. Fecal anti-adhesin IgG and IgA antibody responses were also induced. These findings support the feasibility of TCI for the application of an adhesin-toxin based ETEC vaccine.