Pick a Tag and Explore the Functions of Your Pet Protein. Issue 10 (October 2019)

• Record Type: Journal Article
• Authors: Vandemoortele, Giel; Eyckerman, Sven; Gevaert, Kris
• Is Part Of: Trends in biotechnology. Volume 37:Issue 10(2019)
• Page Start: 1078
• Page End: 1090
• Publication Date: 2019-10
• Subjects: epitope tags -- protein tags -- protein profiling -- CRISPR/Cas9 -- endogenous affinity ligands; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Genetic engineering -- Periodicals; Industrial microbiology -- Periodicals; 660.605
• DOI: 10.1016/j.tibtech.2019.03.016
• Languages: English
• ISSNs: 0167-7799

Abstract

Abstract : Protein tags have been essential for advancing our knowledge of the function of proteins, their localization, and the mapping of their interaction partners. Expressing epitope-tagged proteins has become a standard practice in every life science laboratory and, thus, continues to enable new studies. In recent years, several new tagging moieties have entered the limelight, many of them bringing new functionalities, such as targeted protein degradation, accurate quantification, and proximity labeling. Other novel tags aim at tackling research questions in challenging niches. In this review, we elaborate on recently introduced tags and the opportunities they provide for future research endeavors. In addition, we highlight how the genome-engineering revolution may boost the field of protein tags. Highlights: Genetically encoded tags are continuously being developed and expand the toolbox that protein tags provide. Current applications are centered around purification, protein complex analysis, proximity labeling, quantification, and degradation or localization of the tagged protein. Genome engineering is set to boost the use of protein tags at the endogenous level.