CgSOCS6 negatively regulates the expression of CgIL17s and CgDefh1 in the pacific oyster Crassostrea gigas. Issue 93 (October 2019)

• Record Type: Journal Article
• Authors: Wu, Zhaojun; Sun, Jiejie; Wang, Liyan; Zong, Yanan; Han, Zirong; Yang, Wen; Liu, Zhaoqun; Wang, Lingling; Song, Linsheng
• Is Part Of: Fish & shellfish immunology. Issue 93(2019)
• Page Start: 1084
• Page End: 1092
• Publication Date: 2019-10
• Subjects: Crassostrea gigas -- Suppressor of cytokine signaling 6 -- Immune response -- Interleukin 17 -- Defensin; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.08.055
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: As a family of negatively feedback regulating factors, the suppressor of cytokine signaling (SOCS) can depress cytokine signal transduction, and eventually modulate growth, development, differentiation, and immune response. In the present study, a SOCS homologue (designated as Cg SOCS6) was identified from oyster Crassostrea gigas . The open reading frame of Cg SOCS6 cDNA was of 1167 bp encoding a peptide of 388 amino acid residues with a central Src homology 2 (SH2) domain, a conserved C-terminal SOCS box, and a nucleus localization sequence (NLS) in its N-terminus. The deduced amino acid sequence of Cg SOCS6 shared 37.9–45.5% similarity with other SOCS6/7 family members. In the unrooted phylogenetic tree, Cg SOCS6 was clustered with Es SOCS6 from Chinese mitten crab Eriocheir sinensis and assigned into the SOCS6/7 group. The mRNA transcripts of Cg SOCS6 were constitutively distributed in all the tested tissues, with the highest level in hemocytes. After lipopolysaccharide (LPS) stimulation, the mRNA expression of Cg SOCS6 in hemocytes was significantly up-regulated to the highest level at 6 h (8.48-fold compared to the control group, p < 0.01), and then kept at a relatively higher level from 12 h to 72 h. Cg SOCS6 protein could be translocated into the hemocyte nucleus after LPS stimulation. The mRNA expressions of interleukin 17-4 ( Cg IL17-4), Cg IL17-5, and defensin ( Cg Defh1) in the hemocytes of Cg SOCS6-knockdown oysters increased significantly (2.55-fold, 2.68-fold, 4.68-fold of that in EGFP-RNAi oysters, p < 0.05, p < 0.05, p < 0.001, respectively) after LPS stimulation. These findings suggested that Cg SOCS6 was involved in the oyster immune response by regulating the expressions of Cg IL17-4, Cg IL17-5, and Cg Defh1. Highlights: A conserved SOCS ( Cg SOCS6) was characterized from Crassostrea gigas. The mRNA of Cg SOCS6 was highly expressed in hemocytes and could be induced by LPS. Cg SOCS6 proteins were translocated into nucleus upon LPS stimulation. Cg SOCS6 negatively regulated the expression of Cg IL17-4, Cg IL17-5 and Cg Defh1.