Immunoreactivity of a G protein-coupled l-DOPA receptor GPR143, in Lewy bodies. (November 2019)

• Record Type: Journal Article
• Authors: Goshima, Yoshio; Watanabe, Shuya; Seki, Erika; Koga, Motokazu; Masukawa, Daiki; Nakamura, Fumio; Komori, Takashi; Arai, Nobutaka
• Is Part Of: Neuroscience research. Volume 148(2019)
• Page Start: 49
• Page End: 53
• Publication Date: 2019-11
• Subjects: l-DOPA l-34-dihydroxyphenyalanine -- PD Parkinson's disease -- SN substantia nigra -- SNpc substantia nigra pars compacta -- PBS phosphate buffered saline -- RT room temperature; l-DOPA -- Parkinson's disease -- GPR143 -- Lewy -- Bodies -- α-Synuclein; Neurosciences -- Research -- Periodicals; Neurosciences -- Research -- Japan -- Periodicals; Neurology -- Periodicals; Neurosciences -- Periodicals; Neurosciences -- Recherche -- Périodiques; Neurosciences -- Recherche -- Japon -- Périodiques; Neurosciences -- Research; Japan; Periodicals; 612.8
• DOI: 10.1016/j.neures.2018.12.004
• Languages: English
• ISSNs: 0168-0102

Abstract

Highlights: We generated an antibody raised against human GPR143, al -DOPA receptor. Immunoreactive signals of GPR143 are localized in Lewy bodies. GPR143 is colocalized with phosphorylated α-synuclein at Ser129. Abstract: l -3, 4-Dihydroxyphenylalanine (l -DOPA) has been believed to be an inert amino acid precursor of dopamine, and is the most effective therapeutic agent in Parkinson's disease (PD). We proposedl -DOPA as a neurotransmitter in the central nervous system. Recently, the ocular albinism 1 gene product, OA1/GPR143 (GPR143), was identified as a receptor forl -DOPA. In this study, we examined by generating anti-human GPR143 antibody, the localization of GPR143-immunoreactive signals in the brains from control and PD subjects. GPR143-immunoreactive signals were detected throughout the entire midbrain including substantia nigra pars compacta. In the PD brains, we found that GPR143-immunoreactive signals were detected in Lewy bodies and were colocalized with immunoreactive signals with anti-human Ser129 phosphorylated α-synuclein antibody. Although the significance of its occurrence in the inclusion bodies is unknown, our finding suggests possible implications of GPR143 in PD.