Experimental infection of golden hamsters with Guama virus (Peribunyaviridae, Orthobunyavirus). (October 2019)

• Record Type: Journal Article
• Authors: Matos, Gabriel Costa; Ferreira, Milene Silveira; Martins Filho, Arnaldo Jorge; Amador Neto, Orlando Pereira; Campos, Valter Miranda; Lima, Maria de Lourdes Gomes de; Rodrigues, Jéssica Cecília Pinheiro; Ribeiro, Ana Claudia da Silva; Freitas, Maria Nazaré Oliveira; Silva, Franko de Arruda; Chiang, Jannifer Oliveira; Casseb, Livia Medeiros Neves; Sousa, Jorge Rodrigues de; Quaresma, Juarez Antônio Simões; Martins, Lívia Carício; Vasconcelos, Pedro Fernando da Costa; Carvalho, Valéria Lima
• Is Part Of: Microbial pathogenesis. Volume 135(2019)
• Publication Date: 2019-10
• Subjects: Guama virus -- Golden hamsters -- Experimental infection; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2019.103627
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The Guama virus (GMAV) is a member of Peribunyaviridae family, Orthobunyavirus genus. Several strains of the virus were isolated in South and Central Americas from several hosts, such as humans, wild animals, including nonhuman primates, wild rodents and mosquitoes as well as mice used as sentinels. The virus is able to cause febrile disease in humans. Here we describe for the first time pathologic and biochemical findings in golden hamsters ( Mesocricetus auratus ) infected with the prototype GMAV. Blood and organs of infected and control animals were collected every 24 h after infection from the 1st to the 7th day post infection (dpi) and at 21 dpi when experiment was ended. The tissues were processed for histopathology and immunohistochemistry. The blood and serum were used to determine viremia and biochemical markers plus to detect anti-GMAV antibodies. The viremia was early detected already on the 1st dpi and it was no longer detected on the 3rd dpi. Total anti-GMAV antibodies were detected from the 6th dpi. Hepatic markers as ALT of infected animals were increased and showed statistically significant difference in comparison with control animals, indicating damage of the liver; indeed the liver was the most affected organ, but other organs presented lesions and positive GMAV immunostaining as brain, lung, liver, spleen, and kidney. Our findings indicate that golden hamsters are a good animal model for experimental infection of the GMAV. Highlights: The viremia of hamsters infected by Guama virus (GMAV) was detected on the 1st dpi and it was no longer detected on 3rd dpi. Total anti-GMAV antibodies were detected from the 6th dpi. ALT of animals infected by GMAV were increased and with statistically significant difference compared to the controls. The liver was the most affected organ, but other organs presented lesions and positive GMAV immunostaining. Golden hamsters are a good animal model for experimental infection of the GMAV.