Development of 3D Lymph Node Mimetic for Studying Prostate Cancer Metastasis. Issue 9 (24th July 2019)

• Record Type: Journal Article
• Authors: Hakamivala, Amirhossein; Huang, YiHui; Chang, Yung‐Fu; Pan, Zui; Nair, Ashwin; Hsieh, Jer‐Tsong; Tang, Liping
• Is Part Of: Advanced biosystems. Volume 3:Issue 9(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-07-24
• Subjects: lymph node biosystem -- lymph nodes -- metastasis -- prostate cancer -- T lymphocytes; Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; Biological Science Disciplines; Periodicals; Periodicals; 660.6
• DOI: 10.1002/adbi.201900019
• Languages: English
• ISSNs: 2366-7478

Abstract

Abstract: Lymph node (LN) metastasis causes poor prognosis for patients with prostate cancer (PCa). Although LN‐cells and cellular responses play a pivotal role in cancer metastasis, the interplay between LN‐cells and PCa cells is undetermined due to the small size and widespread distribution of LNs. To identify factors responsible for LN metastasis, a 3D cell culture biosystem is fabricated to simulate LN responses during metastasis. First, it is determined that LN explants previously exposed to high metastatic PCa release substantially more chemotactic factors to promote metastatic PCa migration than those exposed to low‐metastatic PCa. Furthermore, T‐lymphocytes are found to produce chemotactic factors in LNs, among which, CXCL12, CCL21, and IL‐10 are identified to have the most chemotactic effect. To mimic the LN microenvironment, Cytodex beads are seeded with T cells to produce a LN‐mimetic biosystem in both static and flow conditions. As expected, the flow condition permits prolonged cellular responses. Interestingly, when PCa cells with varying metastatic potentials are introduced into the system, it produces PCa‐specific chemokines accordingly. These results support that the LN mimetic helps in analyzing the processes underlying metastasized LNs and for testing various treatments to reduce cancer LN metastasis. Abstract : A 3D Lymph node (LN) mimetic device is developed to simulate the LN environment invaded by cancer cells cancer cell–invading LN environment. This device serves as an excellent tool to determine metastatic potential of cancer, based on the products released from this device.