Modification of genital kidney nephrons for sperm transport in a plethodontid salamander, Eurycea longicauda. (17th July 2019)

• Record Type: Journal Article
• Authors: Hartley, Jordan; Gribbins, Kevin M.; Siegel, Dustin S.
• Is Part Of: Journal of morphology. Volume 280:Number 10(2019)
• Page Start: 1452
• Page End: 1461
• Publication Date: 2019-07-17
• Subjects: histology -- Plethodontidae -- reproduction -- Spelerpini -- Urodela; Morphology -- Periodicals; Physiology -- Periodicals; Anatomy -- Periodicals; 571.3
• DOI: 10.1002/jmor.21041
• Languages: English
• ISSNs: 0362-2525

Abstract

Abstract: Salamanders possess kidneys with two distinct regions: a caudal pelvic portion and cranial genital portion. Nephrons of the pelvic region are responsible for urine formation and transport. Nephrons of the genital region transport sperm from testes to Wolffian ducts; however, nephrons of the genital region possess all the same functional regions found in pelvic kidney nephrons that are involved with urine formation and transport (renal corpuscles, proximal tubules, distal tubules, and collecting ducts). Morphological similarities between pelvic and genital regions stimulated past researchers to hypothesize that nephrons of genital kidneys possess dual function; that is, sperm transport and urine formation/transport. Considering size of glomeruli is directly related to the total amount of blood plasma filtered into the Bowman's space, we tested the hypothesis that nephrons of genital kidneys have reduced urine formation function by comparing glomerular size between nephrons of pelvic and genital kidney regions in Eurycea longicauda with general histological techniques. Light microscopy analysis revealed that glomeruli of pelvic kidneys were significantly larger than those measured from genital kidneys. Transmission electron microscopy analysis also revealed modifications in genital kidney nephrons when compared to pelvic kidney nephrons that suggested a decrease in urine formation function in genital kidneys. Such modifications included a decrease in basal and lateral plasma membrane folding in genital kidney proximal and distal tubules compared to that of pelvic kidney proximal and distal tubules. Genital kidney proximal tubules were also ciliated, which was not observed in pelvic kidney proximal tubules. In conclusion, although structurally similar at the histological level, it appears that nephrons of genital kidneys have decreased urine formation function based on glomerular size comparison and nephron ultrastructure. Abstract : Genital and pelvic kidney glomeruli captured at the same magnification, demonstrating the greater cross sectional surface area of pelvic kidney glomeruli in comparison to genital kidney glomeruli.