Hydrogels: 3D Microenvironment Stiffness Regulates Tumor Spheroid Growth and Mechanics via p21 and ROCK (Adv. Biosys. 9/2019). Issue 9 (9th September 2019)

• Record Type: Journal Article
• Authors: Taubenberger, Anna V.; Girardo, Salvatore; Träber, Nicole; Fischer‐Friedrich, Elisabeth; Kräter, Martin; Wagner, Katrin; Kurth, Thomas; Richter, Isabel; Haller, Barbara; Binner, Marcus; Hahn, Dominik; Freudenberg, Uwe; Werner, Carsten; Guck, Jochen
• Is Part Of: Advanced biosystems. Volume 3:Issue 9(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-09-09
• Subjects: Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; Biological Science Disciplines; Periodicals; Periodicals; 660.6
• DOI: 10.1002/adbi.201970092
• Languages: English
• ISSNs: 2366-7478

Abstract

Abstract : In article number1900128, Anna V. Taubenberger and co‐workers employ fluorescently labelled polyacrylamide beads (Cy3, green) as stress sensors that are incorporated into hydrogels together with breast cancer cells. After 14 days, tumor spheroid cultures were fixed, stained for F‐actin (red) and nuclei (blue), and imaged via confocal microscopy (maximum projection shown). In stiff hydrogels, the beads are deformed in proximity to the spheroid surface, which indicates that the spheroids experience radial compressive stress.