CRISPR-Cas adaptive immunity and the three Rs. Issue 4 (17th July 2017)

• Record Type: Journal Article
• Authors: Killelea, Tom; Bolt, Edward L.
• Is Part Of: Bioscience reports. Volume 37:Issue 4(2017)
• Publication Date: 2017-07-17
• Subjects: CRISPR -- recombination -- replication; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1042/BSR20160297
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : In this summary, we focus on fundamental biology of Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR)-Cas (CRISPR-associated proteins) adaptive immunity in bacteria. Emphasis is placed on emerging information about functional interplay between Cas proteins and proteins that remodel DNA during homologous recombination (HR), DNA replication or DNA repair. We highlight how replication forks may act as 'trigger points' for CRISPR adaptation events, and the potential for cascade-interference complexes to act as precise roadblocks in DNA replication by an invader MGE (mobile genetic element), without the need for DNA double-strand breaks.