Histological, electrophysiological and clinical effects of thermal radiofrequency therapy of the saphenous nerve and pulsed radiofrequency therapy of the sciatic nerve in dogs. (September 2019)

• Record Type: Journal Article
• Authors: Boesch, Jordyn M.; Campoy, Luis; Southard, Teresa; Dewey, Curtis; Erb, Hollis N.; Gleed, Robin D.; Martin-Flores, Manuel; Sakai, Daniel M.; Sutton, Jennifer; Williamson, Baye; Zatroch, Kathryn
• Is Part Of: Veterinary anaesthesia and analgesia. Volume 46:Number 5(2019)
• Page Start: 689
• Page End: 698
• Publication Date: 2019-09
• Subjects: dog -- pain -- radiofrequency -- saphenous nerve -- sciatic nerve -- Wallerian degeneration; Veterinary anesthesia -- Periodicals; 636.089
• DOI: 10.1016/j.vaa.2019.05.006
• Languages: English
• ISSNs: 1467-2987

Abstract

Abstract: Objective: Thermal radiofrequency (TRF) of the saphenous nerve (a sensory nerve) combined with pulsed radiofrequency (PRF) of the sciatic nerve (a sensory and motor nerve) might relieve intractable stifle osteoarthritis (OA) pain in dogs. The objective was to determine if saphenous nerve TRF induces Wallerian degeneration and if sciatic nerve PRF induces degeneration or dysfunction. Study design: Blinded, controlled, randomized, preclinical study. Animals: A group of six intact, female Beagle dogs aged 14–16 months. Methods: In each dog, one pelvic limb was assigned randomly to the control group and the other to the treatment group. Dogs were anesthetized and, using ultrasonography, radiofrequency electrodes were positioned adjacent to saphenous and sciatic nerves bilaterally; TRF and PRF were performed only in the treatment limb. Motor nerve conduction velocity (MNCV) was measured in both sciatic nerves 2 weeks later, and the dogs were euthanized. Hematoxylin and eosin-stained sections of saphenous and sciatic nerves were examined using light microscopy. Degeneration and inflammation were scored 0 (none) to 3 (severe). A one-tailed, paired Wilcoxon signed-rank test was used to test for differences in scores and MNCV between control and treatment nerves. Results: Degeneration and inflammation scores were higher in treatment saphenous nerves in 5/6 dogs [83%; 95% confidence interval (CI), 36%, 99%]; however, after Bonferroni correction only degeneration score was higher ( p = 0.0313). Degeneration, inflammation or decreased MNCV were not observed in sciatic nerves (each outcome: 0/6 nerves, 0%; 95% CI, 0%, 48%). No dogs experienced postprocedural pain or neurological deficits. Conclusions and clinical relevance: The degeneration in TRF-treated saphenous nerves appears sufficient to impair transmission. Sciatic nerve PRF did not cause degeneration with attendant motor deficits, consistent with a proposed neuromodulatory mechanism. A clinical trial is needed to confirm the combined techniques produce analgesia without motor deficits in dogs with stifle OA.